Prevalence of Clostridium perfringens and Clostridium difficile carrying cpa, tcdA toxin genes in raw meat and leafy vegetables

The genus Clostridium is a Gram-positive, spore-forming bacterium. Most species of Clostridium spp. usually are found in soil, water, dead plants, animal carcasses, and play an important role the decomposition of substances nature. Raw meat fresh vegetable more likely to be contaminated with spores or vegetative cells genus Clostridium. study results showed that percentage samples with raw leafy vegetables collected at markets Dong Da, Ha Hoang Mai districts infected C. perfringens was 26.67% (n = 150) 26.82% 220). The percentage raw vegetable difficile was very low 6.00% 0.9% 220), respectively. Gene amplification reactions revealed presence cpa toxin gene all strains detected 02 carrying tcdA gene.